Characterization of an interferon receptor on human lymphoblastoid cells.
نویسندگان
چکیده
منابع مشابه
Production of human lymphoblastoid interferon by Namalva cells.
Optimum conditions for growth and interferon production by a human lymphoblastoid cell line, Namalva, have been studied. Adaptation to large-scale production is possible utilizing either Sendai virus or Newcastle disease virus. Priming of cultures before induction is unnecessary. The interferon produced has properties similar to human leukocyte interferon. The production of lymphoblastoid inter...
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125I-Transferrin binding to lymphoblastoid K562 and Daudi cells markedly increased after exposure of the cells to culture conditions that stimulated proliferation. Treatment of these cells with interferon-alpha (IFN-alpha) resulted in concurrent inhibition of cell growth and of the rise in transferrin binding. Scatchard analyses revealed that IFN reduced the number of transferrin receptors with...
متن کاملCharacterization of human beta-interferon-binding sites on human cells.
Radioiodinated recombinant human beta-interferon (rHuIFN beta Ser), with almost full (greater than 90%) biological activity, was used to study the binding of human beta-interferon to Daudi cells. Specific binding was not observed with less biologically active (less than or equal to 10%) radioiodinated interferon. The bound radioiodinated interferon was shown to compete with human beta-interfero...
متن کاملInterferon-induced gene expression in wild-type and interferon-resistant human lymphoblastoid (Daudi) cells.
Interferon-induced gene expression was analyzed in wild-type and interferon-resistant Daudi cells. Two classes of alpha-interferon-induced mRNAs and proteins were observed: those that were similarly induced in both types of cell and those that were induced only in the wild-type cells. Furthermore, the level of c-myc mRNA decreased in the wild-type but not in the resistant cells. This differenti...
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Protein synthesis in interferon-treated HeLa cells infected by different animal RNA viruses has been studied. Synthesis of vesicular stomatitis virus (VSV) proteins was not detected in cells treated with concentrations of HuIFN-alpha (Ly) above 10 IU/ml. No specific inhibition of glycosylation of the G protein was observed. In addition, inhibition of host protein synthesis in IFN-treated cells ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.11.3269